Adrenergic Receptor Protocols by Curtis A. Machida

By Curtis A. Machida

Oregon well-being Sciences Univ., Beaverton. presents a suite of reproducible equipment for the research of regulators of CNS-mediated habit and neural functionality. For neurobiologists, neurochemists, neurologists, and pharmacologists. DNLM: Receptors, Adrenergic - research.

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Bylund, D. B. (1988) Subtypes of α 2-adrenoceptors: pharmacological and molecular biological evidence converge. Trends Pharmacol. Sci. 9, 356–361. 5. Bylund, D. , Eikenberg, D. , Hieble, J. , Langer, S. , Lefkowitz, R. , Minneman, K. , et al. (1994) IV. International Union of Pharmacology Nomenclature of Adrenoceptors. Pharmacol. Rev. 46, 121–136. 6. Johnson, R. D. and Minneman, K. P. (1987) Differentiation of α1-adrenergic receptors linked to phosphatidylinositol turnover and cyclic AMP accumulation in rat brain.

9. The nonspecific binding and extension of primers prior to the initial denaturation of the template during the first step of PCR can be significantly reduced by keeping the reaction mixes at 0°C before thermal cycling and using “hot-start” techniques (see Note 16). 10. New receptor subtypes or multiple subtypes from species, which have not been rigorously studied, can be determined by designing primers based on two consensus regions from all the members of the same families. However, these Receptor Gene Isolation 11.

In particular, the base composition at the 3′ end should be conserved among all the species or subtypes (see Note 19). 4. Reverse Transcription and the Selection of Primers Several different types of primers and reverse transcriptase (RT) enzymes can be used to form the first-strand or cDNA from either total or poly A+ RNA templates (see Notes 20 and 21). 1. In general, random hexamers are used as primers when a transcript is very long, has a short poly A tail, and/or contains regions with a high % GC content, thus creating areas with significant secondary structures.

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